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Image Search Results
Journal: Human Molecular Genetics
Article Title: Altered keratinocyte differentiation is an early driver of keratin mutation-based palmoplantar keratoderma
doi: 10.1093/hmg/ddz050
Figure Lengend Snippet: Analysis of global gene expression in Krt16-null footpad lesions and comparison to human PC cases. (A) Volcano plot depicting differentially expressed genes in Krt16-null paw skin lesions relative to WT controls. (B) Top 10 genes differentially regulated in Krt16-null paw skin from established paw lesions compared to WT littermate controls. (C) Validation of downregulated genes identified by microarray analysis by RT-qPCR in Krt16-null paw skin lesions. N = 4 mice/genotype. Error bars are SEM. *P < 0.05, **P < 0.01. (D) Overlap in the significantly changed genes that are upregulated between Krt16-null paw skin lesions and KRT16 human cases. (E) Overlap in the significantly changed genes that are downregulated between Krt16-null paw skin lesions and KRT16 human cases. (F) Overlap in the significantly changed genes that are upregulated between Krt16-null paw skin lesions and KRT6 human cases. (G) Overlap in the significantly changed genes that are downregulated between Krt16-null paw skin lesions and KRT6 human cases. Boxes for (D)–(G) list common genes between mouse and human data sets (listed in alphabetical order).
Article Snippet: Total RNA isolated from paw skin, epidermis and dermis, of 2 month old Krt16 -null (2 males, 2 females) and WT (2 males, 2 females) littermates (see Biochemical and morphological analyses ) was subjected to microarray analysis by the
Techniques: Gene Expression, Comparison, Biomarker Discovery, Microarray, Quantitative RT-PCR